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KMID : 0615720120290010075
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Jouranl of Korean Acupuncture and Moxibustion society
2012 Volume.29 No. 1 p.75 ~ p.87
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The Effect of Varying Concentrations of Bee Venom Pharmacoupuncture Treatments on Type II Collagen Induced Arthritis in Mice
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Lee Sung-Woo
Kim Yu-Jong
Kim Eun-Jung
Lee Seung-Deok
Kim Kap-Sung
Yoon Jong-Hwa
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Abstract
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Objectives: The purpose of this study is to inquire into the effect of different concentrations of bee venom pharmacopuncture to inhibit genesis of pro-inflammatory cytokines and to inhibit nuclear factor (NF)-κB activation on type II collagen induced arthritis.
Methods: The experiment was divided into category of the normal group (NOR)-no treated group, control group (CON)-CIA (collagen induced arthritis) induced group, and 4,000 : 1 bee venom group (BV-L)- 4000:1 bee venom pharmacopuncture treated group after CIA, and 2000:1 bee venom group (BV-H)- 2,000 : 1 Bee venom pharmacopuncture treated group after CIA. RA was induced in the mice via injecting 50 ㎕ CⅡ mixed CFA. The bee venom pharmacopuncture was applied on ST35 for 19 days from the 3rd day of RA inducement. To research the effect on the expression of IKK (IκB kinase), iNOS (inducible nitric oxide synthase) & COX-2 (cyclooxygenase-2) mRNA, RT-PCR was performed on synovial membrane cells from the knee joint of CIA mice.
Results: The PMA-induced IκB kinase (IKK), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA expression were dose-dependantly decreased in bee venom treated with synoviocytes. In mice treated with bee venom pharmacopuncture, foot thickness and the damage of synovial membranes of the joint was lessened, and the activation of RA-related pro-inflammatory cytokines such as MIF, TNF-αand MMP-9 was significantly decreased. The activation of iNOS and COX-2 was suppressed by the inhibition of NF-κB. In addition, each data was shown that 2,000 : 1 bee venom pharmacopuncture was more effective than 4,000 : 1 bee venom pharmacopuncture.
Conclusions: It is speculated that bee venom pharmacopuncture has the therapeutic effect of palliating the damage of the synovial membrane and inflammation on RA by suppressing of NF-κB activation.
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KEYWORD
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bee venom, concentration, collagen induced arthritis
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